shrna negative control (shcontrol  (Obio Technology Corp Ltd)

Journal: Oncology Letters

Article Title: Elevated expression of POLD1 is associated with poor prognosis in breast cancer

doi: 10.3892/ol.2018.9392

Figure Lengend Snippet: POLD1 knockdown affects cell cycle progression. (A) Cell cycle distribution of the MCF-7/shControl and MCF-7/shPOLD1 cells was analyzed by flow cytometry. (B) Data for the cell cycle assay results. Data points are presented as the mean ± standard error of the mean. *P<0.01, shControl vs. shPOLD1. POLD1, polymerase δ catalytic subunit gene 1; shPOLD1, short hairpin RNA targeting POLD1; shControl, control short hairpin RNA.

Article Snippet: Short hairpin RNA (shRNA) targeting POLD1 (shPOLD1) and the negative control shRNA (shControl) were purchased from Shanghai GenePharma Co., Ltd. (Shanghai, China). shRNA was designed using online shRNA tools (Invitrogen; Thermo Fisher Scientific, Inc.), and the oligonucleotides encoding POLD1 shRNA were as follows: 5′-CACCGGTCCACCTTCATCCGTATCACGAATGATACGGATGAAGGTGGACC-3′ (forward) and 5′-AAAAGGTCCACCTTCATCCGTATCATTCGTGATACGGATGAAGGTGGACC-3′ (reverse).

Techniques: Flow Cytometry, Cell Cycle Assay, shRNA

Journal: BMC Biochemistry

Article Title: Human POLD1 modulates cell cycle progression and DNA damage repair

doi: 10.1186/s12858-015-0044-7

Figure Lengend Snippet: Modulation of POLD1 expression in HEK293 cells. a qRT-PCR analysis of POLD1 mRNA level in HEK293 cells transfected with pcDNA3.0-POLD1, pcDNA3.0, shPOLD1 or shControl. b Western blot analysis of POLD1 protein level in HEK293 cells transfected with pcDNA3.0-POLD1, pcDNA3.0, shPOLD1 or shControl. Untransfected HEK293 cells were used as a control (Blank). n = 3, * P < 0.01 vs. the negative control (pcDNA3.0 or shControl)

Article Snippet: Short hairpin RNA (shRNA) targeting POLD1 (shPOLD1) and the negative control shRNA (shControl) were purchased from BGI (Shenzhen, China).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Western Blot, Negative Control

Journal: BMC Biochemistry

Article Title: Human POLD1 modulates cell cycle progression and DNA damage repair

doi: 10.1186/s12858-015-0044-7

Figure Lengend Snippet: Effects of altered POLD1 expression on the growth of HEK293 cells. HEK293 cells were transfected with the indicated vectors, and cell proliferation was determined by CCK-8 assay 24, 48, and 72 h later. Data shown were the mean ± SD of the ratio for the absorbance at 450 nm. n = 3, * P < 0.01 vs. shControl

Article Snippet: Short hairpin RNA (shRNA) targeting POLD1 (shPOLD1) and the negative control shRNA (shControl) were purchased from BGI (Shenzhen, China).

Techniques: Expressing, Transfection, CCK-8 Assay

Journal: BMC Biochemistry

Article Title: Human POLD1 modulates cell cycle progression and DNA damage repair

doi: 10.1186/s12858-015-0044-7

Figure Lengend Snippet: Effects of altered POLD1 expression on e cell cycle progression of HEK293 cells. a Representative FACS plots showing cell cycle distribution of HEK293 cells transfected with the indicated vectors. b Quantification of the percentage of cells in different phases. n = 3, * P < 0.01 vs. shControl

Article Snippet: Short hairpin RNA (shRNA) targeting POLD1 (shPOLD1) and the negative control shRNA (shControl) were purchased from BGI (Shenzhen, China).

Techniques: Expressing, Transfection

Journal: BMC Biochemistry

Article Title: Human POLD1 modulates cell cycle progression and DNA damage repair

doi: 10.1186/s12858-015-0044-7

Figure Lengend Snippet: Quantification of DNA synthesis rate in HEK293 cells. a HEK293 cells were transfected with the indicated vectors, 48 h after transfection EdU-positive cells were determined by flow cytometry. b Quantification of the percentage of cells positive for EdU incorporation assay shown in ( a ). n = 3, * P < 0.01 vs. shControl

Article Snippet: Short hairpin RNA (shRNA) targeting POLD1 (shPOLD1) and the negative control shRNA (shControl) were purchased from BGI (Shenzhen, China).

Techniques: DNA Synthesis, Transfection, Flow Cytometry

Journal: BMC Biochemistry

Article Title: Human POLD1 modulates cell cycle progression and DNA damage repair

doi: 10.1186/s12858-015-0044-7

Figure Lengend Snippet: Effects of altered POLD1 expression on DNA damage in HEK293 cells. a HEK293 cells were transfected with the indicated vectors and treated with 150 μM H 2 O 2 for 5 min in the dark. Comet assay was then performed, and images were acquired using a fluorescence microscope to show DNA fragment migration patterns. b Quantification of the tail moments from the comet assay shown in ( a ). * P < 0.01, vs. the negative control (shControl or shControl + H 2 O 2 )

Article Snippet: Short hairpin RNA (shRNA) targeting POLD1 (shPOLD1) and the negative control shRNA (shControl) were purchased from BGI (Shenzhen, China).

Techniques: Expressing, Transfection, Single Cell Gel Electrophoresis, Fluorescence, Microscopy, Migration, Negative Control